Maria was the daughter of Giacomo Tiepolo but her mother is unknown. The date and place of her birth have not been found.
Her husband was Bartolomeo Gradenigo. They were married, but the date and place have not been found. Their six known children were Marco (?-?), Bertucci (?-?), Almoro (?-?), Niccolo (?-?), Giovanni (?-?) and Pietro (?-?).