Anna was the daughter of unknown parents. The date and place of her birth have not been found.
Her partner was Maximilian I von Habsburg, but they were never married. Their nine known children were Max Friedrich (1511-1553), Leopold (1515-1557), Dorothea (1516-1572), Anna (?-?), Anna Margareta (c1517-?), Elisabeth (?-<1584), Barbara (?-?), Cornelius (1507-?) and Christoph Ferdinand (?-?).